Laboratory of plant molecular biology FSc USB
Contact
The laboratory is located at rooms 104-106 in the ground floor of the Building A in the campus of Faculty of Science (Branisovska 1760).
tel.: (38777)2271, (38777)2365 or (38777)2362
Mailing address: Branisovska 1760, Ceske Budejovice 70 05
Contact persons:
Mgr. Jiří Košnar Ph.D. – head of the laboratory, DNA based methods
- e-mail: JiriKosnar@seznam.cz
- tel.: (38777)2271, 2365 – laboratory (or 2303 – Department of Botany)
Mgr. Petr Koutecký Ph.D. – flow cytometry
- e-mail: kouta@prf.jcu.cz
- tel.: (38777)2271, 2362 – laboratoř (or 2377 – Department of Botany)
The laboratory is not a service workplace. The users have to participate on sample processing and follow basic rules of molecular laboratory. Lab form.
Used Methods
- DNA extraction
- PCR (Polymerase Chain Reaction)
- Sanger DNA sequencing
- ISSR (Inter Simple Sequence Repeat)
- SSR (microsatellites)
- PCR-RFLP (Restriction Fragment Lenght Polymorphism)
- AFLP (Amplified Fragment Length Polymorphism)
- Molecular cloning
- library preparation for NGS (different platforms)
- flow cytometry (genome size and ploidy estimation)
Newest protocols available here.
Courses
- Molecular Methods in Plant Science (KBO/309)
- Development of microsatellite markers (SSRs) (KBO/125)
- Flow cytometry in plant biology (KBO/124)
Links
- DNA laboratory at the Department of Botany, Charles University
- Laboratory of Flow Cytometry, Institute of Botany, Průhonice
- Plant Biosystematics Group, Masaryk University, Brno
- Primers – Lutzoni lab (Department of Biology, Duke University, Durham, North Carolina)
- Multiple Primer Analyzer – primer dimer detection, Tm calculation etc. (ThermoFisher Scientific)
- Primer3 – primer design
- MaarjAM database of arbuscular mycorrhizal fungi (AMF)