Laboratory of plant molecular biology FSc USB

Contact

The laboratory is located at rooms 104-106 in the ground floor of the Building A in the campus of Faculty of Science (Branisovska 1760).

tel.: (38777)2271, (38777)2365 or (38777)2362

Mailing address: Branisovska 1760, Ceske Budejovice 70 05

Contact persons:

Mgr. Jiří Košnar Ph.D. – DNA based methods

  • e-mail: JiriKosnar@seznam.cz
  • tel.: (38777)2271, 2365 – laboratory (or 2303 – Department of Botany)

Mgr. Petr Koutecký Ph.D. – isozyme analysis, flow cytometry

  • e-mail: kouta@prf.jcu.cz
  • tel.: (38777)2271, 2362 – laboratoř (or 2377 – Department of Botany)

The laboratory is not a service workplace. The users have to participate on sample processing and follow basic rules of molecular laboratory. Lab form.

Used Methods

  • DNA extraction
  • PCR (Polymerase Chain Reaction)
  • Sanger DNA sequencing
  • ISSR (Inter Simple Sequence Repeat)
  • SSR (microsatellites)
  • PCR-RFLP (Restriction Fragment Lenght Polymorphism)
  • AFLP (Amplified Fragment Length Polymorphism)
  • DNA electrophoresis
  • Isozymes
  • Molecular cloning

Newest protocols available here.

Courses

  • Molecular Methods in Plant Science (KBO/309)
  • Development of microsatellite markers (SSRs) (KBO/125)
  • Flow cytometry in plant biology (KBO/124)

Links